Label-Free Surface-Enhanced Raman Scattering Detection of Fire Blight Pathogen Using a Pathogen-Specific Bacteriophage

• DOI: 10.1021/acs.jafc.3c08217
• Publication Date: 2024-01-22T05:59:09Z
• Authors: Youngho Jeon; Suji Lee; Nguyen Trung Vu; Hyeongsoon Kim; In Sun Hwang; Chang-Sik Oh; Jungmok You
• Keywords: Fire blight

Fire blight is one of the most devastating plant diseases, causing severe social and economic problems. Herein, we report a novel method based on label-free surface-enhanced Raman scattering (SERS) combined with an Erwinia amylovora-specific bacteriophage that allows detecting efficiently fire bacteria E. amylovora for first time. To achieve highest SERS signals amylovora, synthesized compared plasmonic nanoparticles (PNPs) different sizes, i.e., bimetallic gold core–silver shell (Au@AgNPs) monometallic (AuNPs) utilized coffee-ring effect self-assembly PNPs enrichment bacteria. Furthermore, investigated changes in spectra after incubation bacteriophage, found considerable differences as function The results indicate our bacteriophage-based analysis can specifically detect without need peak assignment but simply by monitoring over Therefore, facile holds great potential detection pathogenic investigation viral–bacterial interactions.