Antibacterial Mechanism, Control Efficiency, and Nontarget Toxicity Evaluation of Actinomycin X2 against Xanthomonas citri Subsp. citri

• DOI: 10.1021/acs.jafc.3c08600
• Publication Date: 2024-02-20T22:12:05Z
• Authors: Liangliang Gao; Meiling Huang; Qin Xiong; Yan Liang; Lanfang Mi; Yueming Jiang; Jun Zhang
• Keywords: Citrus canker; Malate dehydrogenase

The present study investigated the antibacterial mechanism, control efficiency, and nontarget toxicity of actinomycin X2 (Act-X2) against Xanthomonas citri subsp. (Xcc) for first time. Act-X2 almost completely inhibited proliferation Xcc in growth curve assay at a concentration 0.25 MIC (minimum inhibitory concentration, = 31.25 μg/mL). This effect was achieved by increasing production reactive oxygen species (ROS), blocking formation biofilms, obstructing synthesis intracellular proteins, decreasing enzymatic activities malate dehydrogenase (MDH) succinate (SDH) Xcc. Molecular docking quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) analysis results indicated that steadily bonded to RNA polymerase, ribosome, dehydrogenase, inhibit their activities, thus drastically reducing expression levels related genes. showed far more effectiveness than commercially available pesticide Cu2(OH)3Cl prevention therapy citrus canker disease. Furthermore, evaluation demonstrated not phytotoxic trees exhibited minimal earthworms both contact soil toxic assays. suggests has potential as an effective environmentally friendly agent.