Cloning, Expression Characteristics of Farnesyl Pyrophosphate Synthase Gene from Platycodon grandiflorus and Functional Identification in Triterpenoid Synthesis

• DOI: 10.1021/acs.jafc.3c09293
• Publication Date: 2024-05-13T12:00:33Z
• Authors: Meiqi Liu; Zhen Wang; Chen Qin; Huiyan Cao; Lingyang Kong; Tingxia Liu; Shan Jiang; Lengleng Ma; Xiubo Liu; Weichao Ren; Wei Ma
• Keywords: Farnesyl pyrophosphate; Cloning (programming); Farnesyl diphosphate synthase; Terpene; Identification; Expression cloning

Platycodon grandiflorus is a medicinal plant whose main component platycodins, which have variety of pharmacological effects and nutritional values. The farnesyl pyrophosphate synthase (FPS) key enzyme in the isoprenoid biosynthesis pathway, catalyzes synthesis diphosphate (FPP). In this study, we cloned FPS gene from P. (PgFPS) with an ORF 1260 bp, encoding 419 amino acids deduced molecular weight theoretical pI 46,200.98 Da 6.52, respectively. squalene content overexpressed PgFPS tobacco leaves yeast cells extract was 1.88-fold 1.21-fold higher than that control group, respectively, total saponin also increased by 1.15 times extract, verified biological function terpenoid synthesis. After 48 h MeJA treatment 6 ethephon treatment, expression roots stems reached its peak, showing 3.125-fold 3.236-fold increase compared to untreated Interestingly, showed decreasing trend after exogenous elicitors treatment. discovery will provide novel perspective for enhancing efficient platycodins.