4-Phenylbutyric Acid Attenuates Soybean Glycinin/β-Conglycinin-Induced IPEC-J2 Cells Apoptosis by Regulating the Mitochondria-Associated Endoplasmic Reticulum Membrane and NLRP-3

• DOI: 10.1021/acs.jafc.3c09630
• Publication Date: 2024-03-08T18:38:18Z
• Authors: Lei Wang; Daoliang Zhang; Benzheng Jiang; Hongyan Ding; Shibin Feng; Chang Zhao; Xichun Wang; Jinjie Wu

Glycinin (11S) and β-conglycinin (7S) from soybean (glycine max) cause diarrhea intestinal barrier damage in young animals. Understanding the mechanisms underlying caused by 7S 11S, it is vital to develop strategies eliminate allergenicity. Consequently, we investigated 7S/11S-mediated apoptosis porcine epithelial (IPEC-J2) cells. IPEC-J2 cells suffered endoplasmic reticulum stress (ERS) response activating protein kinase RNA-like ER kinase, transcription factor 6, C/EBP homologous protein, inositol-requiring enzyme 1 alpha. 4-Phenylbutyric acid (4-PBA) treatment alleviated ERS; reduced NLR family pyrin domain containing 3, interleukin-1β, interleukin-18 levels; inhibited apoptosis; increased mitofusin 2 expression; mitigated Ca2+ overload mitochondria-associated membrane (MAM) dysfunction, thereby ameliorating injury. We demonstrated pivotal role of ERS MAM dysfunction 7S- 11S-mediated apoptosis, providing insights into injury prevention treatment.