Multifaceted Effects of Subchronic Exposure to Chlorfenapyr in Mice: Implications from Serum Metabolomics, Hepatic Oxidative Stress, and Intestinal Homeostasis

• DOI: 10.1021/acs.jafc.3c09682
• Publication Date: 2024-03-19T18:23:41Z
• Authors: Yabing Xiong; Xiaoran Ma; Bingying He; Jianwen Zhi; Xueke Liu; Peng Wang; Zhiqiang Zhou; Donghui Liu
• Keywords: Chlorfenapyr

As chlorfenapyr is a commonly used insecticide in agriculture, the health risks of subchronic exposure to remained unclear. This study aimed extensively probe from at NOAEL and 10-fold dose mice. Through pathological biochemical examinations, body metabolism, hepatic toxicity, intestinal homeostasis were systematically assessed. After 12 weeks, resulted weight reduction, increased daily food intake, blood lipid abnormalities. Concurrently, this dosage induced hepatotoxicity amplified oxidative stress hepatocytes, finding further supported HepG2 cells. Moreover, inflammation, evidenced by inflammatory factors (IL-17a, IL-10, IL-1β, IL-6, IL-22), disrupted immune cells (RORγt, Foxp3), compromised barriers (ZO-1 occludin). By contrast, presented less toxicity most evaluations. Serum metabolomic analyses unveiled widespread disruptions pathways related including NF-κB signaling, Th cell differentiation, bile acid metabolism. Microbiomic analysis showed an increase Lactobacillus, decrease Muribaculaceae, diminished anti-inflammatory microbes, which propelled response leaded inflammation. These findings revealed molecular mechanisms underlying chlorfenapyr-induced highlighting significant role gut microbiota.