Engineering a Carotenoid Cleavage Oxygenase for Coenzyme-Free Synthesis of Vanillin from Ferulic Acid

• DOI: 10.1021/acs.jafc.4c01688
• Publication Date: 2024-05-16T05:56:15Z
• Authors: Rong Zheng; Qihang Chen; Qingbo Yang; Tian Gong; Ching Yuan Hu; Yonghong Meng
• Keywords: Bacillus pumilus; Decarboxylation; Biocatalysis

One-pot biosynthesis of vanillin from ferulic acid without providing energy and cofactors adds significant value to lignin waste streams. However, naturally evolved carotenoid cleavage oxygenase (CCO) with extreme catalytic conditions greatly limited the above pathway for bioproduction. Herein, CCO