(E)-β-Farnesene Protects Rice from Rice Striped Stem Borer Attack by Inhibiting Its Beneficial Gut Microbes

• DOI: 10.1021/acs.jafc.4c02074
• Publication Date: 2024-10-17T15:18:24Z
• Authors: Lei Yang; Pei-tao Zhang; Miao-miao Li; Xin-yu Wang; Qing-qing Zhao; Jun-jie Lin; Jia-qi Zhao; Bao-sheng Liu; Shuai Li; Rui Ji; Ji-chao Fang; Yang Sun
• Keywords: Alkyl and Aryl Transferases; Bacteria; Larva; Animals; Oryza; Herbivory; Moths; Sesquiterpenes; Gastrointestinal Microbiome; Plant Proteins

Herbivores harbor gut microbes that affect their development and contribute to their nutrition, reproduction, and survival. Plant defenses could target the herbivore's beneficial gut microbes, but this has not been well studied in rice. In this context, we identified a rice terpene synthase gene, Os04g0341500. It was strongly induced after feeding by rice striped stem borers (SSB, Chilo suppressalis), and it can catalyze the (E)-β-farnesene (Eβf) synthesis. When added to artificial diets, Eβf impaired the development and survival of SSB larvae. High-throughput amplicon sequencing revealed that SSB fed on Eβf were decreased in beneficial gut microbes, compared to those feeding on the corresponding control feed. In vitro feeding of Eβf suggested that this antimicrobial sesquiterpene directly inhibited the growth of SSB gut microbes. The present study suggested that the Eβf-induced decrease of relative abundance of gut microbes potentially impairs larval development and survival in SSB.