Enhanced Herbicide Metabolism and Target Site Mutation Enabled the Multiple Resistance to Cyhalofop-butyl, Florpyrauxifen-benzyl, and Penoxsulam in Echinochloa crus-galli

• DOI: 10.1021/acs.jafc.4c02450
• Publication Date: 2024-05-08T17:19:22Z
• Authors: Xiaoyue Yu; Jinqiu Sun; Yongjie Yang; Jianping Zhang; Yongliang Lu; Wei Tang
• Keywords: Echinochloa crus-galli

This study investigated the multiple herbicide resistance (MHR) mechanism of one Echinochloa crus-galli population that was resistant to florpyrauxifen-benzyl (FPB), cyhalofop-butyl (CHB), and penoxsulam (PEX). carried an Ala-122-Asn mutation in acetolactate synthase (ALS) gene but no acetyl-CoA carboxylase (ACCase) transport inhibitor response1 (TIR1) genes. The metabolism rate PEX 2-fold higher, production florpyrauxifen-acid cyhalofop-acid lower population. Malathion 4-chloro-7-nitrobenzoxadiazole (NBD-Cl) could reverse resistance, suggesting cytochrome P450 (CYP450) glutathione S-transferase (GST) contribute enhanced metabolism. According RNA-seq qRT-PCR validation, two CYP450 genes (CYP71C42 CYP71D55), GST (GSTT2), glycosyltransferase (rhamnosyltransferase 1 IAAGLU), ABC transporter (ABCG1 ABCG25) were induced by CHB, FPB, revealed target mutant involved MHR E. crus-galli.