Discovery of Antibacterial Compounds against Xanthomonas citri subsp. citri from a Marine Fungus Aspergillus terreus SCSIO 41202 and the Mode of Action

• DOI: 10.1021/acs.jafc.4c02769
• Publication Date: 2024-05-21T16:18:59Z
• Authors: Jun Zhang; Liangliang Gao; Huiting Lin; Yan Liang; Mingnan You; Lijian Ding; Fangjian Feng; Bin Yang; Yonghong Liu
• Keywords: Xanthomonas citri; Aspergillus terreus; Citrus canker; Mode of Action; Diaphorina citri

Citrus canker, caused by Xanthomonas citri subsp. (Xcc), is a severe citrus disease. Currently, copper-containing pesticides are widely used to manage this disease, posing high risks the environment and human health. This study reports discovery of naturally occurring anti-Xcc compounds from deep-sea fungus, Aspergillus terreus SCSIO 41202, possible mode action. The ethyl acetate extract A. was subjected bioassay-guided isolation, resulting in eight (1–8) with minimum inhibitory concentrations (MICs) ranging 0.078 0.625 mg/mL. chemical structures these metabolites were determined integrative analysis various spectroscopic data. Among compounds, Asperporonin A (1) B (2) identified as novel very unusual structural skeleton. electronic circular dichroism determine absolute configurations 1 2 through quantum calculation. bioconversion pathway involving pinacol rearrangement proposed produce (1–2). Compound 6 exhibited an excellent effect MIC value mg/mL, which significantly more potent than positive control CuSO4 (MIC = 0.3125 mg/mL). inhibited cell growth disrupting biofilm formation, destroying membrane, inducing accumulation reactive oxygen species. In vivo tests indicated that compound highly effective controlling canker These results indicate 1–8, especially 6, have potential lead for development new, environmentally friendly, efficient pesticides.