Alternative Method for Glyphosate Determination in Unroasted Green Coffee Beans by Liquid Chromatography Tandem Mass Spectrometry (LC–MS/MS)

• DOI: 10.1021/acs.jafc.4c06366
• Publication Date: 2024-10-24T08:04:16Z
• Authors: Ana Carolina Pere...; Emanuel Carvalho ...; Leonardo d’Antonino; Maria Eliana Lope...; Antonio Alberto d...

This research proposes an alternative method to detect and quantify glyphosate residues in unroasted green coffee beans by LC-MS/MS. The sample preparation was conducted without derivatization steps, with integrated cleanup, which improves the analytical method's frequency. Validation results were consistent with the requirements of the regulatory guidelines employed. Specificity, linearity (r2 = 0.9991), precision (RSD ≤ 9%), and recovery (92-112%) were ensured, with a satisfactory limit of quantification (LOQ = 0.48 mg kg-1). These data demonstrate that the method is suitable for monitoring glyphosate residues in unroasted coffee beans while offering simplicity and speed in sample preparation. The method was applied to analyze authentic unroasted coffee bean samples, in which two of them were contaminated with glyphosate (<LOQ). These results exhibit the importance of glyphosate monitoring in coffee samples and enhance that the method can be successfully implemented as a tool to guarantee food quality and safety.