Integration Site Library for Efficient Construction of Plasmid-Free Microbial Cell Factories in Escherichia coli

• DOI: 10.1021/acs.jafc.4c08290
• Publication Date: 2024-10-26T13:01:05Z
• Authors: Xiaolei Wang; Liangyu Lu; Qiyuan Liu; Jinyi Li; Tong Wang; Jia Wang; Xinxiao Sun; Xiaolin Shen; Qipeng Yuan

Enhanced production stability and efficiency along with a decrease in costs are required to build efficient microbial cell factories. Target genes can be integrated into the genome enhance genetic stability, reduce reliance on antibiotics, alleviate metabolic burden. However, selecting optimal insertion site for desired gene expression levels remains challenging. Therefore, 18 commonly used