Metabolism Plasticity on Account of Aspartate aminotransferase 10 Promotes Poplar Growth under Altered Nitrogen Regimes

• DOI: 10.1021/acs.jafc.4c09107
• Publication Date: 2025-03-06T09:22:32Z
• Authors: Mei Han; Yujia Si; Shuyue Sun; Jinghan Hu; Yirong Han; Xiaoning Liu; Yujie Zhai; Tao Su; Fuliang Cao
• Keywords: Nitrogen Cycle

Improving poplar productivity across a wide spectrum of nitrogen conditions is primary objective in breeding. In this research, we engineered transgenic poplars to overexpress the aspartate aminotransferase 10 (AspAT10) gene. The results showed that these plantlets significantly outperformed wild-type control terms growth under both nitrogen-poor and nitrogen-rich conditions, exhibiting increased biomass, height, root development. This improvement was linked changes internal pools (including NO3-, NH4+, total free amino acids) sugar content. line with metabolic results, notable alterations genes related carbon metabolism as well hormone signaling pathways were identified. Our findings highlight versatile role AspAT10 regulating poplar's adaptation variable availability, attributed reversible nature its catalytic reaction, which allows for flexible reprogramming align supply plant demand.