l-Glutamic Acid Alleviates Mastitis in Dairy Cows by Targeting the Sirt5/Nrf2/Prdx1 Signaling Axis and Mitochondrial Function

• DOI: 10.1021/acs.jafc.4c10238
• Publication Date: 2025-02-03T06:31:35Z
• Authors: Shu Liu; Yuhang Jin; Wenyi Zhou; Juxiong Liu; Liqun Tu; Yufei Zhang; Meng Zhang; Xuanting Liu; Yu Cao; Wenjin Guo; Shoupeng Fu
• Keywords: Glutamic acid

Mastitis in dairy cows is an inflammatory disease that severely affects the health and lactation functions of cows. Mitochondrial damage closely related to response. How effectively alleviate mitochondrial key preventing treating mastitis In this study, we found elevated levels response accompanied by reduced expression Sirt5 (Sirtuin5) with compared healthy This suggests plays important role Subsequently, further analyzed mammary gland tissue from untargeted metabolomics (LC-MS/MS) screened for differential metabolite l-glutamic acid (l-Glu). To validate effect l-Glu on cows, conducted a study using MAC-T cells. The results showed was able ameliorate LPS-induced activating promoting fusion upregulation membrane potential (MMP) levels. contrast, unable protect function after knocking down Sirt5. Furthermore, upregulate nuclear factor E2-related (Nrf2) peroxiredoxin 1 (Prdx1) cells, promoted entry Nrf2 into nucleus, which reversed Next, explored whether alleviates through Nrf2/Prdx1 signaling axis inhibitor RA. use RA blocked protective function. conclusion, ameliorates targeting activate provides new target theoretical basis clinical control mastitis. could be added as dietary supplement diets maintain homeostasis, thereby protecting economic value