Citrinin-Induced Intestinal Onset of Pyroptosis via the IP3R1–GRP75–VDAC1 Complex-Mediated Mitochondrial Oxidative Stress

• DOI: 10.1021/acs.jafc.4c11218
• Publication Date: 2025-02-26T01:50:26Z
• Authors: Yuanyuan Li; Qike Zhang; Xiaofang Liu; Yongkang Wang; Chenglin Yang; You Wu; Bo Xiao; Yiya Feng; Aoao Wu; Jine Yi; Jing Wu; Zengenni Liang; Zhihang Yuan
• Keywords: Citrinin; Pyroptosis

Citrinin (CTN) is commonly found in animal feed and stored grains poses a serious threat to human health. Formation of the IP3R1–GRP75–VDAC1 complex has been shown play key role intestinal defense against harmful stimuli, but mechanism its action CTN-exposure-induced enterotoxicity not clear. Therefore, aim this study was investigate IPEC-J2 monolayer cell damage mice. It that CTN exposure triggered pyroptosis increased formation as well mitochondrial levels calcium ions reactive oxygen species (mtROS). And mtROS considered be factor cellular pyroptosis. removal by using Mito-Tempo attenuate failed ion overload. However, silencing GRP75 alleviated increases level mtROS, ions, subsequent confirms induces juxtaposition tissues points out oxidative stress mediated which triggers