Functional Analysis of Lactase Phlorizin Hydrolase in Insect–Plant Coevolution Based on Deglycosylation

• DOI: 10.1021/acs.jafc.4c12035
• Publication Date: 2025-02-24T16:16:37Z
• Authors: Yuan Li; Wenting Kong; Tingting Li; Lianjun Zhang; Ziyan Zhuang; Ning Liu; Xiaoning Liu
• Keywords: Lactase

During coevolution, plants produce numerous toxic secondary metabolites to protect themselves. However, most still serve as food for insects meet their nutritional needs. The evolutionary processes that enable herbivorous resist plant defenses remain largely complex and difficult predict. In this study, lactase phlorizin hydrolase (LPH) was identified the first time in Aphis gossypii. Bioinformatics analysis showed LPH protein belongs glycoside 1 (GH1) family. qPCR transcript level of cotton aphids treated with expression dsAgCYP6CY3 lines significantly reduced by 39.9% at 48 h compared nontransgenic cotton, hydrolytic activities on lactose were 28.7% 20.1%, respectively. vitro enzyme activity experiments demonstrated could hydrolyze four phenolic glycosides. addition, RNA interference (RNAi) insect performance assays silencing affected growth larvae, resulting death larvae exposed These results reveal an scenario whereby herbivores use deglycosylation develop resistance can be exploited protection.