Yellow Pigment Aurovertins Mediate Interactions between the Pathogenic Fungus Pochonia chlamydosporia and Its Nematode Host

• DOI: 10.1021/acs.jafc.5b02595
• Publication Date: 2015-07-07T16:01:51Z
• Authors: Yan-li Wang; Lin-fang Li; Dong-xian Li; Baile Wang; Keqin Zhang; Xuemei Niu
• Keywords: Nematode infection; Root-knot nematode; Xenorhabdus

Nematophagous fungi are globally distributed soil and well-known natural predators of soil-dwelling nematodes. Pochonia chlamydosporia can be found in diverse nematode-suppressive soils as a parasite nematode eggs is one the most studied potential biological control agents However, little known about functions small molecules process infection nematodes by this parasitic fungus or small-molecule-mediated interactions between pathogenic its host. Our recent study demonstrated that P. strain isolated from root knots tobacco infected root-knot Meloidogyne incognita produced class yellow pigment metabolite aurovertins, which induced death free-living Panagrellus redivevus. Here we report nematicidal strains obtained worms tended to yield total aurovertin production exceeding inhibitory concentration shown bioassays. Aurovertin D was abundant metabolites strains. showed strong toxicity toward M. exerted profound detrimental effects on viability Caenorhabditis elegans even at subinhibitory concentration. Evaluation mutation β subunit F1-ATPase, together with application RNA interference screening each F1FO-ATPase worms, F1-ATPase might not specific target for aurovertins The resistance C. daf-2(e1370) hypersensitivity daf-16(mu86) indicated DAF-16/FOXO transcription factor triggered response attack. These findings advance our understanding roles pathogen chlamydosporia.