Plant Esterase–Chitosan/Gold Nanoparticles–Graphene Nanosheet Composite-Based Biosensor for the Ultrasensitive Detection of Organophosphate Pesticides

• DOI: 10.1021/acs.jafc.5b03971
• Publication Date: 2015-11-10T21:08:27Z
• Authors: Jing Bao; Changjun Hou; Mei Chen; Junjie Li; Danqun Huo; Mei Yang; Xiaogang Luo; Yu Lei
• Keywords: Paraoxon; Nanosheet; Parathion methyl; Tap water

As broad-spectrum pesticides, organophosphates (OPs) are widely used in agriculture all over the world. However, due to their neurotoxicity humans and increasing occurrence environment, there is growing interest sensitive selective detection. This paper reports a new cost-effective plant esterase–chitosan/gold nanoparticles–graphene nanosheet (PLaE-CS/AuNPs-GNs) biosensor for detection of methyl parathion malathion. Highly pure esterase produced from plants at low cost shares same inhibition mechanism with OPs as acetylcholinesterase, then it was prepare PLaE-CS/AuNPs-GNs nanocomposites, which were systematically characterized using SEM, TEM, UV–vis. The composite-based measured 50 ppt (0.19 nM) 0.5 ppb (1.51 malathion (S/N = 3) calibration curve up 200 (760 500 (1513.5 malathion, respectively. There also no interference observed most common species such metal ions, inorganic glucose, citric acid. In addition, its applicability OPs-contaminated real samples (carrot apple) demonstrated excellent response recovery. developed simple, sensitive, reliable holds great potential food environmental safety.