Combination of Silver Nanoparticles and Curcumin Nanoparticles for Enhanced Anti-biofilm Activities

• DOI: 10.1021/acs.jafc.5b04559
• Publication Date: 2015-11-23T13:38:58Z
• Authors: Ching-Yee Loo; Ramin Rohanizadeh; Paul M. Young; Daniela Traini; Rosalia Cavaliere; Cynthia B. Whitch...; Wing-Hin Lee
• Keywords: Silver nanoparticle

Biofilm tolerance has become a serious clinical concern in the treatment of nosocomial pneumonia owing to resistance various antibiotics. There is an urgent need develop alternative antimicrobial agents or combination drug therapies that are effective via different mechanisms. Silver nanoparticles (AgNPs) have been developed as anti-biofilm agent for infections associated with use mechanical ventilations, such endotracheal intubation. Meanwhile curcumin, phenolic plant extract, displayed natural properties through inhibition bacterial quorum sensing systems. The aim this study was investigate possible synergistic/additive interactions AgNPs and curcumin (Cur-NPs) against both Gram-negative (Pseudomonas aeruginosa) Gram-positive (Staphylococcus aureus) microorganisms. Cur-NPs (termed Cur-SNPs) at 100 μg/mL disrupted 50% established biofilms (formed on microtiter plates). However, further increase concentration Cur-SNPs failed effectively eliminate biofilms. To achieve same effect, least 500 Cur-NP alone needed. Scanning electron microscopy (SEM) confocal laser scanning (CLSM) revealed therapy (Cur-SNPs) most potent eradicate preformed biofilm compared monodrug therapy. These also nontoxic healthy human bronchial epithelial cells (BEAS2B).