Quantitation of Thioprolines in Grape Wine by Isotope Dilution–Liquid Chromatography–Tandem Mass Spectrometry

• DOI: 10.1021/acs.jafc.5b05604
• Publication Date: 2016-01-23T16:17:36Z
• Authors: Jingjing Liu; Xiangpeng Meng; Wan Chan
• Keywords: Isotope dilution

Cysteine reacts with reactive carbonyls to form thioprolines, which have been demonstrated possess various pharmaceutical properties. Therefore, thioproline formation is considered as a major detoxification pathway for carcinogenic carbonyls. In this study, we report the initial identification of thiazolidine-4-carboxylic acid (1) and 2-methylthiazolidine-4-carboxylic (2), two very common formed by reacting formaldehyde acetaldehyde cysteine in grape wine samples. We developed an isotope dilution–liquid chromatography–tandem mass spectrometry method featuring high sensitivity (limit detection ≤1.5 ng/mL) selectivity quantitate compounds 1 2. The after validated be highly accurate (recovery ≥92%) precise [intraday relative standard deviation (RSD) ≤4.1% interday RSD ≤9.7%] was applied determine varying compound 2 contents Results revealed type storage duration-dependent thioprolines wines. Overall, results are expected facilitate compound-dependent investigations health benefits wine, our findings could adopted predict age wine.