Purification and Characterization of Plantaricin JLA-9: A Novel Bacteriocin against Bacillus spp. Produced by Lactobacillus plantarum JLA-9 from Suan-Tsai, a Traditional Chinese Fermented Cabbage

• DOI: 10.1021/acs.jafc.5b05717
• Publication Date: 2016-03-17T15:56:46Z
• Authors: Shengming Zhao; Jinzhi Han; Xiaomei Bie; Zhaoxin Lu; Chong Zhang; Fengxia Lv
• Keywords: Chromatography, Reverse-Phase; Pyridines; Bacillus; Brassica; 04 agricultural and veterinary sciences; Hydrogen-Ion Concentration; 6. Clean water; Anti-Bacterial Agents; 3. Good health; Molecular Weight; Lactobacillus; 0404 agricultural biotechnology; Bacteriocins; 13. Climate action; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Fermentation; Gram-Negative Bacteria; Chymotrypsin; Amino Acid Sequence; Picolinic Acids; 0405 other agricultural sciences; Lactobacillus plantarum

Bacteriocins are ribosomally synthesized peptides with antimicrobial activity produced by numerous bacteria. A novel bacteriocin-producing strain, Lactobacillus plantarum JLA-9, isolated from Suan-Tsai, a traditional Chinese fermented cabbage, was screened and identified its physiobiochemical characteristics 16S rDNA sequence analysis. new bacteriocin, designated plantaricin purified using butanol extraction, gel filtration, reverse-phase high-performance liquid chromatography. The molecular mass of JLA-9 shown to be 1044 Da MALDI-TOF-MS analyses. amino acid predicted FWQKMSFA MALDI-TOF-MS/MS, which confirmed Edman degradation. This bacteriocin exhibited broad-spectrum antibacterial against Gram-positive Gram-negative bacteria, especially Bacillus spp., high thermal stability (20 min, 121 °C), narrow pH (pH 2.0–7.0). It sensitive α-chymotrypsin, pepsin, alkaline protease, papain. mode action this responsible for outgrowth inhibition cereus spores studied. Plantaricin had no detectable effects on germination initiation over 1 h monitoring the hydration, heat resistance, 2,6-pyridinedicarboxylic (DPA) release spores. Rather, is prerequisite JLA-9. inhibited growth preventing establishment oxidative metabolism disrupting membrane integrity in germinating within 2 h. results suggest that has potential applications control spp. food industry.