Novel Cholecystokinin Secretion-Stimulating Peptides from Oat Protein Hydrolysate: Sequence Identification and Insight into the Mechanism of Action

• DOI: 10.1021/acs.jafc.5c00727
• Publication Date: 2025-04-25T07:48:01Z
• Authors: Hongdong Song; Lei Xue; Qiuyun Fu; Haiyan Wang; Hongwei Cao; Kai Huang; Xiao Guan
• Keywords: Mechanism of Action; Identification; Sequence (biology)

Cholecystokinin (CCK), secreted by enteroendocrine cells, plays a vital role in suppressing appetite. This study aimed to evaluate the vivo effect of oat protein hydrolysate (OPH) on CCK secretion and elucidate structural characteristics responsible peptides their underlying mechanism action. OPH was prepared simulated gastrointestinal digestion model. Intragastric administration mice significantly increased plasma levels. Using size exclusion column chromatography liquid chromatography-tandem mass spectrometry, ten were successfully identified, abilities stimulate evaluated STC-1 cells. Four novel secretion-stimulating peptides, including LLL, QQVFQPQ, QGDVVALPA, DVNNNANQLEPR, validated. Among them, QGDVVALPA exhibited strongest activity. Inhibition experiments demonstrated that calcium-sensing receptor its coupled G-protein subtype Gq involved QGDVVALPA-stimulated secretion. Additionally, downstream signaling molecules intracellular Ca2+ Ca2+/CaM-dependent kinase (CaMKII) also required for induce Our findings highlight potential protein-derived as functional food ingredients regulate satiety.