Antiallergic Activity of Ethanol Extracts of Arctium lappa L. Undried Roots and Its Active Compound, Oleamide, in Regulating FcεRI-Mediated and MAPK Signaling in RBL-2H3 Cells

• DOI: 10.1021/acs.jafc.6b00425
• Publication Date: 2016-04-18T04:47:49Z
• Authors: Woong-Suk Yang; Sung Ryul Lee; Yong Joon Jeong; Dae Won Park; Young Mi Cho; Hae Mi Joo; Inhye Kim; Young-Bae Seu; Eun-Hwa Sohn; Se Chan Kang
• Keywords: Arctium lappa; Active compound

The antiallergic potential of Arctium lappa L. was investigated in Sprague–Dawley rats, ICR mice, and RBL-2H3 cells. Ethanol extract (90%) A. (ALE, 100 μg/mL) inhibited the degranulation rate by 52.9%, determined level β-hexosaminidase. ALE suppressed passive cutaneous anaphylaxis (PCA) rats attenuated histamine release mice. To identify active compound ALE, we subsequently fractionated β-hexosaminidase all subfractions. Oleamide identified as an which secretion production tumor necrosis factor (TNF)-α interleukin-4 (IL-4) cells treated with 48/80 or A23187/phorbol myristate acetate (PMA). FcεRI-tyrosine kinase Lyn-mediated pathway, c-Jun N-terminal kinases (JNK/SAPK), p38 mitogen-activated protein (p38-MAPKs). These results showed that oleamide allergic reactions should serve a platform to search for compounds activity.