Discovery and LC-MS Characterization of New Crocins in Gardeniae Fructus and Their Neuroprotective Potential

• DOI: 10.1021/acs.jafc.6b03866
• Publication Date: 2016-12-10T11:11:35Z
• Authors: Yang Ni; Lin Li; Weiyang Zhang; Dan Lu; Caixia Zang; Dan Zhang; Yang Yu; Xinsheng Yao
• Keywords: 0301 basic medicine; 03 medical and health sciences

Ten new crocins, neocrocins B-J (1-9) and 13-cis-crocetin-8'-O-β-d-gentiobioside (14), along with 10 known were isolated from the fruits of Gardenia jasminoides Ellis (Gardeniae Fructus). The structures these compounds elucidated by analyzing HRESIMS, UV/vis, 1D 2D NMR spectra, their neuroprotective effects against hydrogen peroxide- l-glutamic acid-induced SH-SY5Y cell injury evaluated. UPLC-Q/TOF-MS chromatogram a crocin-rich fraction derived gardenia fruit extracts was established using obtained crocin as references. Most peaks identified (the total integral area accounted for 95% peak areas), bioactive crocins large portion this areas 70% total).