Effects of Wet-Blending on Detection of Melamine in Spray-Dried Lactose

• DOI: 10.1021/acs.jafc.7b00834
• Publication Date: 2017-05-24T22:58:07Z
• Authors: Betsy Jean Yakes; Marti M. Bergana; Peter F. Scholl; Magdi M. Mossoba; Sanjeewa R. Karun...; Luke K. Ackerman; Jason D. Holton; Boyan Gao; Jeffrey C. Moore
• Keywords: Milk; 0404 agricultural biotechnology; Triazines; Animals; Cattle; Food Contamination; Lactose; 04 agricultural and veterinary sciences; Powders; 0405 other agricultural sciences; Food Analysis

During the development of rapid screening methods to detect economic adulteration, spray-dried milk powders prepared by dissolving melamine in liquid exhibited an unexpected loss characteristic features near-infrared (NIR) and Raman spectra. To further characterize this "wet-blending" phenomenon, lactose samples were produced as a simplified model investigated NIR spectroscopy, proton nuclear magnetic resonance (1H NMR), direct analysis real time Fourier transform mass spectrometry (DART-FTMS). In contrast dry-blended samples, bands spectra disappeared or shifted wet-blended lactose-melamine samples. Subtle shifts 1H NMR between wet- indicated differences hydrogen-bonding status. Qualitative DART-FTMS detected greater relative abundance condensation product ions which supported hypothesis that wet-blending facilitates early Maillard reactions Collectively, these data formation weak, H bonded complexes labile, reaction products contribute spectral observed powder These results have implications for future evaluations adulterated emphasize important role sample preparation on adulterant detection.