Selective Detection of Shiga-like Toxin 1 from Complex Samples Using Pigeon Ovalbumin Functionalized Gold Nanoparticles as Affinity Probes

• DOI: 10.1021/acs.jafc.7b00863
• Publication Date: 2017-05-11T18:43:12Z
• Authors: Chun-Hsien Li; Yi-Ling Bai; Karuppuchamy Selv...; Kwok-Kong Tony Mong; Yu-Chie Chen
• Keywords: 0301 basic medicine; Ovalbumin; Swine; Food Contamination; Biosensing Techniques; Escherichia coli O157; Shiga Toxin 1; Shiga Toxin 2; 3. Good health; Meat Products; 03 medical and health sciences; Animals; Nanoparticles; Gold; Columbidae

Escherichia coli O157:H7 is a foodborne pathogen. This bacterial strain can generate Shiga-like toxins (SLTs), which cause serious sickness and even death. Thus, it important to develop effective sensitive methods that be used rapidly identify the presence of SLTs from complex samples. Pigeon egg white (PEW) contains abundant glycoproteins, including pigeon ovalbumin (POA) (∼60%). POA possesses Gal-α(1→4)-Gal-β(1→4)-GlcNAc termini, recognize B subunits in SLT type 1 (SLT-1B). suitable probe for trapping SLT-1B. In this work, we PEW proteins as starting materials react with aqueous tetrachloroauric acid generation PEW-protein-immobilized gold nanoparticles (AuNPs@PEW) via one-pot reactions. We demonstrated generated AuNPs@PEW were mainly dominated by POA-immobilized Au NPs. The as-prepared affinity probes selectively SLT-1B cell lysates derived E. O157:H7. selective step completed within ∼90 s under microwave heating (power = 450 W) enrich sufficient matrix-assisted laser desorption/ionization (MALDI) mass spectrometric analysis. Furthermore, approach detect at concentration low ∼40 pM. feasibility using proposed method ham contaminated was also demonstrated.