Metabolic Profile of Zearalenone in Liver Microsomes from Different Species and Its in Vivo Metabolism in Rats and Chickens Using Ultra High-Pressure Liquid Chromatography-Quadrupole/Time-of-Flight Mass Spectrometry

• DOI: 10.1021/acs.jafc.7b04663
• Publication Date: 2017-12-05T12:15:47Z
• Authors: Shupeng Yang; Huiyan Zhang; Feifei Sun; Karl De Ruyck; Jinzhen Zhang; Yue Jin; Yanshen Li; Zhanhui Wang; Suxia Zhang; Sarah De Saeger; Jinhui Zhou; Yi Li; Marthe De Boevre
• Keywords: Hydroxylation; Metabolic pathway; Biotransformation

To explore differences of zearalenone (ZEN) metabolism between various species, phase I and II by liver microsomes animals human were investigated using ultra high-pressure liquid chromatography-quadrupole/time-of-flight mass spectrometry (UHPLC-Q/TOF MS). A total 24 metabolites identified, among which 12 reported for the first time. Reduction, hydroxylation, glucuronidation major metabolic pathways ZEN, significant in species also observed. Reduction was main reaction swine human, whereas hydroxylation predominant rats, chickens, goats, cows vitro systems. Furthemore, vivo ZEN rats chickens investigated, 23 6 identified each respectively. while reduction sulfation predominated chickens. These results further enrich biotransformation profile providing a helpful reference assessing risks to humans.