Isoproturon-Induced Salicylic Acid Confers Arabidopsis Resistance to Isoproturon Phytotoxicity and Degradation in Plants

• DOI: 10.1021/acs.jafc.8b04281
• Publication Date: 2018-11-08T00:49:43Z
• Authors: Feng Fan Lu; Jiang Yan Xu; Li Ya Ma; Xiang Ning Su; Xin Qiang Wang; Hong Yang
• Keywords: 0301 basic medicine; 03 medical and health sciences; Arabidopsis Proteins; Herbicides; Tandem Mass Spectrometry; Phenylurea Compounds; Arabidopsis; Salicylic Acid; Chromatography, High Pressure Liquid; Herbicide Resistance

This study identified the effect of salicylic acid on degradation of isoproturon in Arabidopsis. Three T-DNA insertion mutant lines pal1- 1, pal1- 2, and eps1- 1 defective in salicylic acid synthesis were tested, which showed higher isoproturon accumulation and a toxic symptom in the mutants. When treated with 5 mg/L salicylic acid, these lines displayed a lower level of isoproturon and showed an attenuated toxic symptom. An RNA-sequencing study identified 2651 (1421 up and 1230 down) differentially expressed genes (DEGs) in eps1- 1 and 2211 (1556 up and 655 down) in pal1- 2 mutant plants (>2.0 fold change, p < 0.05). Some of the DEGs covered Phase I-III reaction components, like glycosyltransferases (GTs) and ATP-binding cassette transporters (ABCs). Using ultra performance liquid chromatography-time-of-flight-tandem-mass spectrometer/mass spectrometer (UPLC/Q-TOF-MS/MS), 13 Phase I and four Phase II metabolites were characterized. Of these, two metabolites 1-OH-isopropyl-benzene-O-glucoside and 4-isopropylphenol-S-2-methylbutanoyl-serine, have been identified and reported for the first time.