Active Components from Sea Buckthorn (Hippophae rhamnoides L.) Regulate Hepatic Stellate Cell Activation and Liver Fibrogenesis

Liver Cirrhosis Male 0301 basic medicine 0303 health sciences Plant Extracts Apoptosis Actins Rats Rats, Sprague-Dawley 03 medical and health sciences Liver Transforming Growth Factor beta Fruit Hippophae Hepatic Stellate Cells Animals Humans DNA Damage Signal Transduction
DOI: 10.1021/acs.jafc.8b05306 Publication Date: 2018-11-06T12:27:13Z
ABSTRACT
Sea buckthorn ( Hippophae rhamnoides L.) is a berry bearing multiple nutritional properties. In this study, 46 compounds were isolated from sea buckthorn berries. Preliminary data showed that the components, C13, C15, and C32, exhibited profound inhibitory effect on the activation of hepatic stellate cells (HSCs) induced by transforming growth factor-β (TGF-β) and decreased the levels of inflammatory factors. Furthermore, these compounds over-regulated the proteins of DNA damage signaling pathway and alpha-smooth muscle actin (α-SMA). Moreover, active components of sea buckthorn berry (ACSB) treatment attenuated fibrosis development in rats after bile duct ligation (BDL), reducing liver injury and inflammation, and reviving liver function in a dose-dependent manner. Moreover, ACSB down-regulated the expression of α-SMA, while over-regulating the DNA damage signaling pathway and the related genes. These suggest that ACSB inhibit DNA repair of HSCs, make them in a damaged state, inhibit the expression of TGF-β, and induce apoptosis.
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