Active Components from Sea Buckthorn (Hippophae rhamnoides L.) Regulate Hepatic Stellate Cell Activation and Liver Fibrogenesis
Liver Cirrhosis
Male
0301 basic medicine
0303 health sciences
Plant Extracts
Apoptosis
Actins
Rats
Rats, Sprague-Dawley
03 medical and health sciences
Liver
Transforming Growth Factor beta
Fruit
Hippophae
Hepatic Stellate Cells
Animals
Humans
DNA Damage
Signal Transduction
DOI:
10.1021/acs.jafc.8b05306
Publication Date:
2018-11-06T12:27:13Z
AUTHORS (3)
ABSTRACT
Sea buckthorn ( Hippophae rhamnoides L.) is a berry bearing multiple nutritional properties. In this study, 46 compounds were isolated from sea buckthorn berries. Preliminary data showed that the components, C13, C15, and C32, exhibited profound inhibitory effect on the activation of hepatic stellate cells (HSCs) induced by transforming growth factor-β (TGF-β) and decreased the levels of inflammatory factors. Furthermore, these compounds over-regulated the proteins of DNA damage signaling pathway and alpha-smooth muscle actin (α-SMA). Moreover, active components of sea buckthorn berry (ACSB) treatment attenuated fibrosis development in rats after bile duct ligation (BDL), reducing liver injury and inflammation, and reviving liver function in a dose-dependent manner. Moreover, ACSB down-regulated the expression of α-SMA, while over-regulating the DNA damage signaling pathway and the related genes. These suggest that ACSB inhibit DNA repair of HSCs, make them in a damaged state, inhibit the expression of TGF-β, and induce apoptosis.
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