Active Components from Sea Buckthorn (Hippophae rhamnoides L.) Regulate Hepatic Stellate Cell Activation and Liver Fibrogenesis

• DOI: 10.1021/acs.jafc.8b05306
• Publication Date: 2018-11-06T12:27:13Z
• Authors: Guokun Zhang; Yifei Liu; Ping Liu
• Keywords: Hippophae rhamnoides; Hepatic stellate cell; Steatosis

Sea buckthorn ( Hippophae rhamnoides L.) is a berry bearing multiple nutritional properties. In this study, 46 compounds were isolated from sea berries. Preliminary data showed that the components, C13, C15, and C32, exhibited profound inhibitory effect on activation of hepatic stellate cells (HSCs) induced by transforming growth factor-β (TGF-β) decreased levels inflammatory factors. Furthermore, these over-regulated proteins DNA damage signaling pathway alpha-smooth muscle actin (α-SMA). Moreover, active components (ACSB) treatment attenuated fibrosis development in rats after bile duct ligation (BDL), reducing liver injury inflammation, reviving function dose-dependent manner. ACSB down-regulated expression α-SMA, while over-regulating related genes. These suggest inhibit repair HSCs, make them damaged state, TGF-β, induce apoptosis.