Anthocyanins Extracted from Aronia melanocarpa Protect SH-SY5Y Cells against Amyloid-beta (1–42)-Induced Apoptosis by Regulating Ca2+ Homeostasis and Inhibiting Mitochondrial Dysfunction

• DOI: 10.1021/acs.jafc.8b05404
• Publication Date: 2018-11-11T16:07:35Z
• Authors: Lingshuai Meng; Guang Xin; Bin Li; Dongnan Li; Xiyun Sun; Tingcai Yan; Li Li; Lin Shi; Sen Cao; Xianjun Meng
• Keywords: Membrane Potential, Mitochondrial; 0301 basic medicine; 0303 health sciences; Amyloid beta-Peptides; Gene Expression; Apoptosis; 01 natural sciences; 0104 chemical sciences; Mitochondria; 3. Good health; Anthocyanins; Neuroblastoma; 03 medical and health sciences; Adenosine Triphosphate; Neuroprotective Agents; Alzheimer Disease; Cell Line, Tumor; Photinia; Homeostasis; Humans; Calcium

We investigated the cytoprotective effects of anthocyanins in Aronia melanocarpa against apoptosis induced by Aβ1-42, a key mediator AD pathophysiology. measured intracellular calcium with colorimetric kit, cellular DAPI, ROS fluorescent marker 2,3-dimethoxy-1,4-naphthoquinone, mitochondrial membrane potential JC-1, and ATP kit. Gene transcription protein expression levels calmodulin, cytochrome c, caspase-9, cleaved caspase-3, Bcl-2, Bax were analyzed RT-PCR Western blotting. The results showed that pretreatment significantly inhibited Aβ1-42-induced apoptosis, decreased ROS, increased potential. blotting revealed upregulated gene calmodulin Bcl-2 downregulated those Bax. A. protected SH-SY5Y cells regulating Ca2+ homeostasis apoptosis-related genes inhibiting dysfunction.