Genome-Wide DNA Methylome and Transcriptome Analysis of Porcine Intestinal Epithelial Cells upon Deoxynivalenol Exposure

• DOI: 10.1021/acs.jafc.9b00613
• Publication Date: 2019-04-23T19:53:17Z
• Authors: Haifei Wang; Qiufang Zong; Shiqin Wang; Chengxiang Zhao; Shenglong Wu; Wenbin Bao
• Keywords: Differentially methylated regions; Epigenomics; Pseudogene

Deoxynivalenol (DON) is a type of mycotoxin that disruptive to intestinal and immune systems. To better understand the molecular effects DON exposure, we performed genome-wide comparisons DNA methylation gene expression from porcine epithelial cell IPEC-J2 upon exposure using reduced representation bisulfite sequencing RNA-seq technologies. We characterized pattern changes found 3030 differentially methylated regions. Moreover, 3226 genes showing differential were enriched in pathways protein nucleic acid synthesis ribosome biogenesis. Integrative analysis identified 29 inverse correlations between promoter expression. Altered various suggested their roles potential functional interactions exposure. Our data provided new insights into epigenetic transcriptomic alterations cells may advance identification biomarkers drug targets for predicting controlling toxic this common mycotoxin.