Characterization of Microalgal Acetyl-CoA Synthetases with High Catalytic Efficiency Reveals Their Regulatory Mechanism and Lipid Engineering Potential

• DOI: 10.1021/acs.jafc.9b03370
• Publication Date: 2019-08-06T10:22:26Z
• Authors: Tao Wu; Xuemei Mao; Yaping Kou; Yuelian Li; Han Sun; Yongjin He; Feng Chen
• Keywords: Characterization

Acetyl-CoA synthetase (ACS) plays a key role in microalgal lipid biosynthesis and acetyl-CoA industrial production. In the present study, two ACSs were cloned characterized from oleaginous microalga Chromochloris zofingiensis. vitro kinetic analysis showed that Km values of CzACS1 CzACS2 for potassium acetate 0.99 0.81 mM, respectively. Moreover, had outstanding catalytic efficiencies (kcat/Km), which 70.67 79.98 s–1 mM–1, respectively, these higher than other reported ACSs. exhibited differential expression patterns at transcriptional level under various conditions. Screening recombinant library 52 transcription factors (TFs) constructed study via yeast one-hybrid assay pointed to seven TFs with potential involvement regulation ACS genes. Expression correlation implied GATA20 was likely an important regulator ERF9 could regulate CzACSs simultaneously.