Pyridine Alkaloids in the Venom of Imported Fire Ants

• DOI: 10.1021/acs.jafc.9b03631
• Publication Date: 2019-09-19T20:46:45Z
• Authors: Jian Chen; Yang Zhao; Xing-Cong Li; Jin-Hao Zhao
• Keywords: Fire ant; Piperidine

Venomous imported fire ants cause significant medical problems. Alkaloids are an important component of ant venom. Piperidine and piperideine alkaloids have been identified in In this study, we studied the venom red ant, Solenopsis invicta Buren, black richteri Forel, hybrid, S. × richteri. Pyridine were detected first time using solid-phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (SPME-GC-MS). The thermal desorption process was manipulated to facilitate isolation identification pyridine that coeluted piperidine or GC. After SPME extraction venom, conducted a series consecutive GC-MS injections, each partial desorption. Hidden alkaloid peaks revealed after overlapping piperidiene had desorbed. Using approach, 10 2-methyl-6-alkyl (or alkenyl)pyridines (1-10) found ants. Structures three confirmed by synthesis, including 2-methyl-6-undecylpyridine (1), 2-methyl-6-tridecylpyridine (7), 2-methyl-6-pentadecylpyridine (10). We also developed silica gel column chromatography method separate from other alkaloids. single ion monitoring at 107 m/z, five quantified for both workers female alates