Recycling Strategy and Repression Elimination for Lignocellulosic-Based Farnesene Production with an Engineered Escherichia coli

0301 basic medicine Waste Products 0106 biological sciences Hydrolysis Lignin Zea mays 01 natural sciences 0104 chemical sciences 12. Responsible consumption 3. Good health 03 medical and health sciences Glucose Metabolic Engineering Fermentation Escherichia coli Recycling Sesquiterpenes 0105 earth and related environmental sciences
DOI: 10.1021/acs.jafc.9b03907 Publication Date: 2019-08-07T09:13:22Z
ABSTRACT
Farnesene is an important chemical platform for many industrial products, such as biofuels and polymers. We performed high-efficiency utilization of corncobs for β-farnesene production by separate hydrolysis and fermentation with an optimized Escherichia coli strain. First, we developed a recycling strategy for both corncob pretreatment and cellulose hydrolysis, which saved great amounts of pretreatment reagents and presented a 96.83% cellulose conversion rate into glucose. However, the corncob hydrolysate strongly repressed cell growth and β-farnesene production, being caused by high-concentrated citrate. Through expressing a heterologous ATP citrate lyase and screening for a suitable expression host, an optimized strain was constructed that produced β-farnesene at 4.06 g/L after 48 h in a 5 L fermenter, representing an approximately 2.3-fold increase over the initial strain. Therefore, the proposed strategy about the recycling process and repression elimination was successful and suitable for the production of lignocellulosic-based β-farnesene, which can be further studied to scale up for industrialization.
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