Alleviated Antibiotic-Resistant Genes in the Rhizosphere of Agricultural Soils with Low Antibiotic Concentration

• DOI: 10.1021/acs.jafc.9b06634
• Publication Date: 2020-01-29T17:50:58Z
• Authors: Mengke Song; Ke Peng; Longfei Jiang; Dayi Zhang; Dandan Song; Guoen Chen; Huijuan Xu; Yongtao Li; Chunling Luo
• Keywords: Crops, Agricultural; 2. Zero hunger; 0303 health sciences; Bacteria; Agriculture; 15. Life on land; Anti-Bacterial Agents; Soil; 03 medical and health sciences; Bacterial Proteins; Drug Resistance, Bacterial; Rhizosphere; Soil Microbiology

The influence of the rhizosphere on the abundance and diversity of antibiotic resistance genes (ARGs) has been recognized but there is a lack of consensus because of broad ranges of plant species and antibiotic concentrations across different habitats and the elusive underlying mechanisms. Here, we profiled antibiotic concentrations and resistomes in the rhizosphere and bulk soils by cultivating 10 types of crops in manure-amended agricultural soils. Rhizosphere effects altered the antibiotic resistome structure, significantly increased the absolute abundance of the antibiotic resistome, and decreased their relative abundance, contrasting previous studies. Such plantation-driven variation in ARGs resulted from the boost of bacterial lineages with negative relationships with ARGs and the constraint of the potential ARG-hosts in the rhizosphere of plants cultivated in soils with low antibiotic concentrations as the selective pressure. This mechanism is not reported previously and deepens our understanding about the rhizosphere effects on ARGs.