Development of a Microscope Stage with Light-Emitting Diodes to Upgrade a Traditional Microscope to a Fluorescence Microscope

• DOI: 10.1021/acs.jchemed.2c00036
• Publication Date: 2022-05-19T17:00:14Z
• Authors: Haruka Honda; Noritaka Miyamoto; Hisayuki Miyajima; Yuichi Tanaka
• Keywords: Inverted microscope

Fluorescence microscopy is a useful tool that allows students to learn the principles of fluorescence visually in chemical and biological fields. However, equipment has high purchase maintenance costs thus may not be available educational institutions or insufficiently funded laboratories. In this study, microscope stage with colored light-emitting diodes (LEDs) was designed enabled conversion traditional inverted into view reducing these costs. A 3D printer used produce 12 holes on back side, standard LED mounted each hole. To evaluate performance proposed system, Hoechst 33342- fluorescein diacetate-stained fish-derived PLHC-1 cells were observed using an stage. Subsequently, images captured, they exhibited bright fluorescence. This system can assembled easily operated by teachers, researchers, even students. Therefore, it learning resource for detection laboratories are well-funded.