Location of the High-Affinity Mn2+ Site in Photosystem II Detected by PELDOR

• DOI: 10.1021/acs.jpcb.5b03994
• Publication Date: 2015-07-23T15:56:32Z
• Authors: Mizue Asada; Hiroyuki Mino
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; 13. Climate action; 3. Good health

The location of the high-affinity Mn(2+) site in apo-photosystem (PS) II was investigated by pulsed EPR. electron-electron magnetic dipole interaction 1.7 MHz between YD(•) radical and ion observed using double resonance (PELDOR) technique, bound to one apo-PS absence presence Ca(2+). PELDOR signals were calculated previously determined spin distribution on its known position crystal structure, assuming that specific located oxygen evolving complex. results show is at denoted Mn4(A) native structure. coordinated with axial ligands Asp170 Glu333 D1 polypeptide.