Investigating the Interaction of Silicon Dioxide Nanoparticles with Human Hemoglobin and Lymphocyte Cells by Biophysical, Computational, and Cellular Studies

• DOI: 10.1021/acs.jpcb.8b00193
• Publication Date: 2018-03-14T18:37:02Z
• Authors: Negin Sabziparvar; Yosra Saeedi; Mina Nouri; Atefeh Sadat Naja...; Elahe Alizadeh; Farnoosh Attar; Keivan Akhtari; Seyyedeh Elaheh M...; Mojtaba Falahati
• Keywords: Acridine orange; Ethidium bromide; Docking (animal); Silicon dioxide; Viability assay; MTT assay

Nanoparticles (NPs) have received great attention in biological and medical applications because of their unique features. However, induced adverse effects on the system are not well-explored. Herein, interaction silicon dioxide nanoparticles (SiO2 NPs) with human hemoglobin (Hb) lymphocyte cell line was evaluated under physiological conditions by multispectroscopic [intrinsic synchronous fluorescence spectroscopy circular dichrosim (CD)], molecular docking, cellular [3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) acridine orange/ethidium (AO/EB) staining] methods. Transmission electron microscopy dynamic light scattering revealed nanosized spherical shaped SiO2 particle. The lifetime decay results indicated that NPs quenched intrinsic intensity Hb through a static quenching mechanism. binding affinity toward directly correlated temperature. sign thermodynamic parameters demonstrated hydrophobic forces played pivotal role Hb. experiments displayed Tyr residues moved to more hydrophilic microenvironment. Molecular docking studies exhibited Si were bound primarily residues. findings from CD data verified no alteration secondary structure upon NPs. treated at varying concentrations time intervals cytotoxicity assays MTT AO/EB staining showed viability reduced NP-induced apoptosis mechanism dose time-dependent manner. Therefore, it may be suggested comprehensive details regarding systems such as cells proteins can provide useful information development NP-based systems.