Cellular Imprinting Proteomics Assay: A Novel Method for Detection of Neural and Ocular Disorders Applied to Congenital Zika Virus Syndrome

• DOI: 10.1021/acs.jproteome.0c00320
• Publication Date: 2020-07-19T14:03:16Z
• Authors: Livia Rosa-Fernandes; Raquel Hora Barbosa; Maria Luiza B. do...; Claudia B. Angeli; Thiago P. Silva; Rossana C. N. Melo; Gilberto Santos d...; Bernardo Lemos; Jennifer E Van Eyk; Martin R. Larsen; Claudete Araújo C...; Giuseppe Palmisano
• Keywords: Zika Virus; Microcephaly

Congenital Zika syndrome was first described due to increased incidence of congenital abnormalities associated with virus (ZIKV) infection. Since the eye develops as part embryo central nervous system (CNS) structure, it becomes a specialized compartment able display symptoms neurodegenerative diseases and has been proposed noninvasive approach early diagnosis neurological diseases. Ocular lesions result from defects that occurred during embryogenesis can become apparent in newborns exposed ZIKV. Furthermore, absence microcephaly cannot exclude occurrence ocular other CNS manifestations. Considering need for surveillance infants possible exposure, we developed method termed cellular imprinting proteomic assay (CImPA) evaluate surface proteome specific ZIKV gestation compared nonexposure. CImPA combines cells fluid capture using membrane disks large-scale quantitative proteomics approach, which allowed first-time report molecular alterations such neutrophil degranulation, cell death signaling, pathways, are infection without development syndrome, CZS. Particularly, clinical could be detected method. Lastly, this methodology broad applicability translated study several identify novel diagnostic biomarkers. Data available via ProteomeXchange identifier PXD014038.