MicroPOTS Analysis of Barrett’s Esophageal Cell Line Models Identifies Proteomic Changes after Physiologic and Radiation Stress

• DOI: 10.1021/acs.jproteome.0c00629
• Publication Date: 2021-01-25T19:24:54Z
• Authors: Kenneth Weke; Ashita Singh; Naomi Uwugiaren; Javier A. Alfaro; Tongjie Wang; Ted R. Hupp; J. Robert O’Neill; Borek Vojtesek; David R. Goodlett; Sarah M. Williams; Mowei Zhou; Ryan T. Kelly; Ying Zhu; Irena Dapic
• Keywords: S100A9

Moving from macroscale preparative systems in proteomics to micro- and nanotechnologies offers researchers the ability deeply profile smaller numbers of cells that are more likely be encountered clinical settings. Herein a recently developed microscale proteomic method, microdroplet processing one pot for trace samples (microPOTS), was employed identify changes ∼200 Barrett's esophageal following physiologic radiation stress exposure. From this small population cells, microPOTS confidently identified >1500 protein groups, achieved high reproducibility with Pearson's correlation coefficient value R > 0.9 over 50% overlap replicates. A cell line model treated either lithocholic acid (LCA) or X-ray had 21 (e.g., ASNS, RALY, FAM120A, UBE2M, IDH1, ESD) 32 GLUL, CALU, SH3BGRL3, S100A9, FKBP3, AGR2) overexpressed proteins, respectively, compared untreated set. These results demonstrate routinely quantify differentially expressed proteins limited cells.