Comparison of Microflow and Analytical Flow Liquid Chromatography Coupled to Mass Spectrometry Global Metabolomics Methods Using a Urea Cycle Disorder Mouse Model

• DOI: 10.1021/acs.jproteome.1c00628
• Publication Date: 2021-11-29T20:10:01Z
• Authors: Sarah Geller; Harvey Lieberman; Adam J. Belanger; Nelson S. Yew; Alla Kloss; Alexander R. Ivanov
• Keywords: Flow injection analysis; Sample Preparation; Repeatability; Chemometrics

Microscale-based separations are increasingly being applied in the field of metabolomics for analysis small-molecule metabolites. These methods have potential to provide improved sensitivity, less solvent waste, and reduced sample-size requirements. Ion-pair free microflow-based global methods, which we recently reported, were further compared analytical flow ion-pairing reagent containing using a sample set from urea cycle disorder (UCD) mouse model. Mouse urine brain homogenate samples representing healthy, diseased, disease-treated animals analyzed by both methods. Data processing was performed univariate multivariate techniques followed analyte trend analysis. The microflow comparably with ability separate three groups when partial least-squares number detected metabolic features present after each data step similar between negative ionization mode. observed coverage known UCD biomarkers same evaluated approaches. 12.5-fold reduction injection volume required highlights this method support studies limitations.