Carbon Metabolism and the ROS Scavenging System Participate in Nostoc flagelliforme’s Adaptive Response to Dehydration Conditions through Protein Acetylation

• DOI: 10.1021/acs.jproteome.1c00823
• Publication Date: 2022-01-12T19:46:12Z
• Authors: Lingxia Wang; Xiaoxu Li; Meng Wang; Xiaorong Ma; Fan Song; Jinhong Hu; Wangli Liang; Wenyu Liang
• Keywords: KEGG; Metabolic pathway

Acetylation represents an extensively occurring protein post-translational modification (PTM) that plays a key role in many cellular physiological and biochemical processes. However, studies on PTMs such as acetylation of lysine (LysAc) cyanobacteria are still rare. In this study, quantitative LysAc approach (acetylome) the strains Nostoc flagelliforme subjected to different dehydration treatments was conducted. We observed starch contents were significantly accumulated due treatments, we identified 2474 acetylpeptides 1060 acetylproteins based acetylome analysis. Furthermore, integrative analysis performed nontargeted metabolism, results showed KEGG terms overlapped for both omics analyses, including sucrose transporter activity, carbon metabolism. addition, time series clustering analyzed, some proteins related metabolism ROS scavenging system enriched list differentially abundant (DAAPs). These expression levels further tested by qPCR. A working model finally proposed show biological roles from response N. flagelliforme. highlighted essential regulation metabolic enzymes stress response.