Prevaccination Glycan Markers of Response to an Influenza Vaccine Implicate the Complement Pathway

• DOI: 10.1021/acs.jproteome.2c00251
• Publication Date: 2022-06-27T07:40:37Z
• Authors: Rui Qin; Guanmin Meng; Smruti Pushalkar; Michael A. Carlock; Ted M. Ross; Christine Vogel; Lara K. Mahal
• Keywords: 0301 basic medicine; 03 medical and health sciences; Glycosylation; Influenza Vaccines; Polysaccharides; Influenza, Human; Humans; Proteins; Antibodies, Viral; Mannose; 3. Good health

A key to improving vaccine design and vaccination strategy is understand the mechanism behind variation of response with host factors. Glycosylation, a critical modulator immunity, has no clear role in determining responses. To gain insight into association between glycosylation vaccine-induced antibody levels, we profiled pre- postvaccination serum protein glycomes 160 Caucasian adults receiving FLUZONE influenza during 2019-2020 season using lectin microarray technology. We found that prevaccination levels Lewis antigen (Lea) are significantly higher nonresponders than responders. Glycoproteomic analysis showed Lea-bearing proteins enriched complement activation pathways, suggesting potential tuning activities proteins, which may be implicated mounting In addition, observed increase sialyl X (sLex) decrease high mannose glycans among responders, were not nonresponders. These data suggest immune system actively modulate as part its effort establish effective protection postvaccination.