Integrative Metabolomics and Proteomics Allow the Global Intracellular Characterization of Bacillus subtilis Cells and Spores

• DOI: 10.1021/acs.jproteome.3c00386
• Publication Date: 2024-01-08T20:32:36Z
• Authors: Yixuan Huang; Bhagyashree N. Sw...; Winfried Roseboom; Jurre D. Bleeker; Stanley Brul; Peter Setlow; Gertjan Kramer
• Keywords: Proteomics; Spores, Bacterial; 570; Ethanol; Methanol; 610; Water; Bacillus subtilis

Reliable and comprehensive multi-omics analysis is essential for researchers to understand explore complex biological systems more completely. Bacillus subtilis (B. subtilis) a model organism Gram-positive spore-forming bacteria, in-depth insight into the physiology molecular basis of spore formation germination in this requires advanced multilayer data sets generated from same sample. In study, we evaluated two monophasic methods polar nonpolar compound extraction (acetonitrile/methanol/water; isopropanol/water, 60% ethanol) biphasic (chloroform/methanol/water, methyl tert-butyl ether/methanol/water) on coefficients variation analytes, identified metabolite composition, quality proteomics profiles. The EtOH protocol proved be easiest sample processing was amenable automation. Collectively, annotated 505 484 metabolites 1665 1562 proteins B. vegetative cells spores, respectively. We also show differences between spores perspective demonstrate that an integrative can implemented one using protocol. results obtained by provide metabolic protein makeup spores.