Analysis of Vascular Smooth Muscle Cells from Thoracic Aortic Aneurysms Reveals DNA Damage and Cell Cycle Arrest as Hallmarks in Bicuspid Aortic Valve Patients

• DOI: 10.1021/acs.jproteome.3c00649
• Publication Date: 2024-04-10T04:17:22Z
• Authors: Ariadna Martin-Bl...; Marta Martin-Lorenzo; Aranzazu Santiago...; Angeles Heredero; Alicia Donado; Juan A Lopez; Miriam Anfaiha-Sa...; Rocio Ruiz-Jimenez; Vanesa Esteban; Jesus Vazquez; Gonzalo Aldamiz-E...; Gloria Alvarez-Ll...
• Keywords: Thoracic aortic aneurysm

Thoracic aortic aneurysm (TAA) is mainly sporadic and with higher incidence in the presence of a bicuspid valve (BAV) for unknown reasons. The lack drug therapy to delay TAA progression lies limited knowledge pathophysiology. We aimed identify molecular hallmarks that differentiate dilatation associated BAV tricuspid (TAV). Aortic vascular smooth muscle cells (VSMCs) isolated from patients or TAV were analyzed by mass spectrometry. DNA oxidative damage assay cell cycle profiling performed three independent cohorts supporting proteomics data. alteration secreted proteins was confirmed plasma. Stress phenotype, stress, enhanced response (increased S-phase arrest apoptosis) found BAV-TAA patients. increased levels plasma C1QTNF5, LAMA2, THSB3, FAP confirm stress BAV-TAA. Plasma BGN point an inflammatory condition TAV. arterial wall shows capacity counteract drivers TAA. pathways identified support need differential diagnosis therapeutic approaches patients, showing specific markers which may serve monitor efficacy.