Comprehensive Proteomics Analysis of the Hemolymph Composition of Sugar-Fed Aedes aegypti Female and Male Mosquitoes

• DOI: 10.1021/acs.jproteome.3c00918
• Publication Date: 2024-03-05T14:28:55Z
• Authors: Patricia H. Alvar...; Thiago Luiz Alves...; Motoshi Suzuki; Glenn Nardone; Pedro Cecilio; Joel Vega-Rodriguez; Jose M.C. Ribeiro; John F. Andersen
• Keywords: Hemolymph; Proteome; Blood meal

In arthropods, hemolymph carries immune cells and solubilizes transports nutrients, hormones, other molecules that are involved in diverse physiological processes including immunity, metabolism, reproduction. However, despite such importance, little is known about its composition. We applied mass spectrometry-based label-free quantification approaches to study the proteome of perfused from sugar-fed female male Aedes aegypti mosquitoes. A total 1403 proteins were identified, out which 447 them predicted be extracellular. both sexes, almost half these extracellular defense/immune response, their relative abundances (based on intensity-based absolute quantification, iBAQ) 37.9 33.2%, respectively. Interestingly, among them, 102 serine proteases/serine protease-homologues with containing CLIP regulatory domains. Moreover, belonging families classically described as chemoreceptors, odorant-binding (OBPs) chemosensory (CSPs), also highly abundant sexes. Our data provide a comprehensive catalogue A. basal protein content, revealing numerous unexplored targets for future research mosquito physiology disease transmission. It provides reference studies effect blood meal infection