Altered Metabolism during the Dark Period in Drosophila Short Sleep Mutants

• DOI: 10.1021/acs.jproteome.4c00106
• Publication Date: 2024-06-05T15:41:45Z
• Authors: Dania M. Malik; Seth D. Rhoades; Pinky Kain; Arjun Sengupta; Amita Sehgal; Aalim M. Weljie
• Keywords: Sleep; Wild type

Sleep is regulated via circadian mechanisms, but effects of sleep disruption on physiological rhythms, in particular metabolic cycling, remain unclear. To examine this question, we probed diurnal alterations two Drosophila short mutants, fumin and sleepless. Samples were collected with high temporal sampling (every 2 h) over 24 h under a 12:12 light:dark cycle, profiling was done using an ion-switching LCMS/MS method. Fewer metabolites oscillations noted (50 46 sleepless, BH. Q < 0.2 by RAIN analysis) compared to wild-type control (iso31, 63 0.2), peak phases the mutants consolidated into major phase peaks at mid-day middle night. Overall, altered nicotinate/nicotinamide, alanine/aspartate/glutamate, acetylcholine, glyoxylate/dicarboxylate, TCA cycle metabolism observed indicative increased energetic demand oxidative stress wild type. Both changes cycling discriminant models suggest unique dark period constrained networks. Thus, conclude that loss alters function uniquely throughout day, further examination specific mechanisms warranted.