Exploring Infantile Epileptic Spasm Syndrome: A Proteomic Analysis of Plasma Using the Data-Independent Acquisition Approach

• DOI: 10.1021/acs.jproteome.4c00298
• Publication Date: 2024-06-10T15:50:10Z
• Authors: Haohua Huang; Dezhi Cao; Yan Hu; Qianqian He; Xia Zhao; Li Chen; Sufang Lin; Xufeng Luo; Yuanzhen Ye; Jianxiang Liao; Huafang Zou; Dongfang Zou
• Keywords: KEGG

This study aimed to identify characteristic proteins in infantile epileptic spasm syndrome (IESS) patients' plasma, offering insights into potential early diagnostic biomarkers and its underlying causes. Plasma samples were gathered from 60 patients with IESS 40 healthy controls. Data-independent acquisition proteomic analysis was utilized differentially expressed (DEPs). These DEPs underwent functional annotation through Gene Ontology (GO) the Kyoto Encyclopedia of Genes Genomes (KEGG) pathway enrichment analyses. set (GSEA) employed for both GO (GSEA-GO) KEGG (GSEA-KEGG) analyses examine gene expression profiles. Receiver operating (ROC) curves assessed biomarkers' discriminatory capacity. A total 124 identified mainly linked pathways, encompassing chemokines, cytokines, oxidative detoxification. GSEA-GO GSEA-KEGG indicated significant genes associated cell migration, focal adhesion, phagosome pathways. ROC curve demonstrated that combination PRSS1 ACTB, PRSS3, alone exhibited AUC values exceeding 0.7. elucidated contribution detoxification, phagosomes pathogenesis. The ACTB holds promise as diagnosis IESS.