Comparative Proteomics Provides Insights into Metabolic Responses in Rat Liver to Isolated Soy and Meat Proteins

• DOI: 10.1021/acs.jproteome.5b00922
• Publication Date: 2016-02-17T17:38:23Z
• Authors: Shangxin Song; Guido J. Hooiveld; Wei Zhang; Mengjie Li; Fan Zhao; Jing Zhu; Xinglian Xu; Michael Muller; Chunbao Li; Guanghong Zhou
• Keywords: Proteome; Fatty Acid Metabolism; Protein metabolism

It has been reported that isolated dietary soy and meat proteins have distinct effects on physiology liver gene expression, but the impact protein expression responses are unknown. Because these may differ from responses, we investigated protein-induced changes in proteome. Rats were fed for 1 week semisynthetic diets differed only regarding source; casein (reference) was fully replaced by soy, chicken, fish, or pork protein. Changes proteome measured iTRAQ labeling LC–ESI–MS/MS. A robust set totaling 1437 unique identified subjected to differential analysis biological interpretation. Compared with casein, all other sources reduced abundance of involved fatty acid metabolism Pparα signaling pathway. All proteins, except increased oxidoreductive transformation reactions energy essential amino metabolic pathways. Only sulfur-containing nonessential acids. Soy fish translation mRNA processing, whereas chicken TCA cycle immune responses. These findings partially line previously transcriptome results. This study further shows rats.