Dynamic Changes in the Protein Localization in the Nuclear Environment in Pancreatic β-Cell after Brief Glucose Stimulation

• DOI: 10.1021/acs.jproteome.7b00930
• Publication Date: 2018-03-08T23:46:52Z
• Authors: Taewook Kang; Pia Jensen; Vita Solovyeva; Jonathan R. Brewer; Martin R. Larsen
• Keywords: Nuclear pore

Characterization of molecular mechanisms underlying pancreatic β-cell function in relation to glucose-stimulated insulin secretion is incomplete, especially with respect global response the nuclear environment. We focus on characterization proteins environment β-cells after brief, high glucose stimulation. compared purified nuclei derived from stimulated 17 mM for 0, 2, and 5 min using quantitative proteomics, a time frame that most likely does not result translation new protein cell. Among differentially regulated proteins, we identified 20 components organization processes, including pore organization, ribonucleoprotein complex, pre-mRNA transcription. found alteration together calcium/calmodulin-binding chaperones facilitate RNA import or export to/from nucleus cytoplasm. Putative mRNA transcription-associated factors were among they cross-validated by Western blotting confocal immunofluorescence imaging. Collectively, our data suggest translocation between cytoplasm an important process, highly involved initial mechanism β-cells.