Supplementation of Lactobacillus plantarum Improves Markers of Metabolic Dysfunction Induced by a High Fat Diet

• DOI: 10.1021/acs.jproteome.8b00282
• Publication Date: 2018-06-22T10:07:20Z
• Authors: Alice Martinic; Javad Barouei; Zach Bendiks; Darya Mishchuk; Dustin D. Heeney; Roy Martin; Maria L. Marco; Carolyn M. Slupsky
• Keywords: Cecum

Obesity is a prevalent chronic condition in many developed and developing nations that raises the risk for heart disease, stroke, diabetes. Previous studies have shown consuming particular probiotic strains of Lactobacillus associated with improvement obese diabetic phenotype; however, mechanisms these beneficial effects are not well understood. In this study, C57BL/6J male mice were fed lard-based high fat diet 15 weeks plantarum supplementation NCIMB8826 (Lp) between 10 (n = per group). Systemic metabolic analyzed by NMR metabolomics, protein expression assays, gene transcript quantification, 16S rRNA marker sequencing. Body organ weights significantly different Lp supplementation, no microbiota community structure changes observed cecum; L. numbers increased treatment group according to culture-based quantification. Significant differences metabolite concentrations (serum, liver, colon), (ileum adipose), cytokines (colon) groups increases tight junction proteins ileum cecum some markers glucose homeostasis blood tissue supplementation. These results indicate impacts systemic metabolism immune signaling before phenotypic without large-scale microbiome. This study supports notion probiotic, even context diet.